Anticorps Recombinant de lapin anti-CKAP2L

CKAP2L Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250860G10

N° de cat : 86247-1-PBS

Synonymes

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

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Galerie de données de validation

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  WB analysis using 86247-1-RR (same clone as 86247-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86247-1-RR (CKAP2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 86247-1-RR (same clone as 86247-1-PBS)

  IP result of anti-CKAP2L (IP:86247-1-RR, 4ug; Detection:86247-1-RR 1:1000) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86247-1-RR (same clone as 86247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP2L antibody (86247-1-RR, Clone: 250860G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86247-1-PBS cible CKAP2L dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CKAP2L Protéine recombinante Ag10909
• Nom complet: cytoskeleton associated protein 2-like
• Masse moléculaire calculée: 745 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC036217
• Symbole du gène: CKAP2L
• Identification du gène (NCBI): 150468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).

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