Anticorps Polyclonal de lapin anti-CKB/CKM

CKB/CKM Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15137-1-AP

Synonymes

B CK, CKB, CKB/CKM, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain

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Galerie de données de validation

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  WB analysis using 15137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15137-1-AP (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 15137-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15137-1-AP (Creatine kinase B type antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15137-1-AP

  IP Result of anti-Creatine kinase B type (IP:15137-1-AP, 4ug; Detection:15137-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 15137-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15137-1-AP (CKB/CKM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 15137-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15137-1-AP (CKB/CKM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de côlon de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

15137-1-AP cible CKB/CKM dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKB/CKM Protéine recombinante Ag7285
• Nom complet: creatine kinase, brain
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001190
• Symbole du gène: CKB
• Identification du gène (NCBI): 1152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.

Publications

Species	Application	Title
human	WB	Cell Metab; Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.; Authors - Kiran Kurmi
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
mouse	WB,IF	Toxicol Appl Pharmacol; Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.; Authors - Yi Lu
rat	WB	Cell Death Dis; Exosomes derived from cardiac progenitor cells attenuate CVB3-induced apoptosis via abrogating the proliferation of CVB3 and modulating the mTOR signaling pathways.; Authors - Xin Li
human	WB	Anticancer Res; Comparative Proteomic Profiling of Cisplatin-resistant Nasopharyngeal Carcinoma Cell Lines: Novel Biomarkers for Improving Chemotherapy of NPC.; Authors - Parisa Prathaphan