Anticorps Monoclonal anti-CKB/CKM

• Phare
• Validé par KD/KO

CKB/CKM Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B7C3

N° de cat : 66764-1-PBS

Synonymes

CKB, KCRB, 2B7C3, B CK, B-CK

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Galerie de données de validation

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  WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66764-1-Ig (same clone as 66764-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66764-1-PBS cible CKB/CKM dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CKB/CKM Protéine recombinante Ag7933
• Nom complet: creatine kinase, brain
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001190
• Symbole du gène: CKB
• Identification du gène (NCBI): 1152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.

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