Anticorps Polyclonal de lapin anti-CKM/CKB

CKM/CKB Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15891-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15891-1-AP (same clone as 15891-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 15891-1-AP (same clone as 15891-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 15891-1-AP (same clone as 15891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15891-1-PBS cible CKM/CKB dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKM/CKB Protéine recombinante Ag8678
• Nom complet: creatine kinase, muscle
• Masse moléculaire calculée: 381 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC007462
• Symbole du gène: CKM
• Identification du gène (NCBI): 1158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.

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