Anticorps Polyclonal de lapin anti-CKM-Specific

CKM-Specific Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18712-1-AP

Synonymes

CKMM, Creatine Kinase MM, Creatine Kinase M-Specific, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK

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Galerie de données de validation

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  WB analysis using 18712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18712-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 18712-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP (CKM-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18712-1-AP (CKM-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18712-1-AP (CKM-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart using 18712-1-AP (CKM-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat heart using 18712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart using 18712-1-AP (CKM-Specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

18712-1-AP cible CKM-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris, Bactérie
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: creatine kinase, muscle
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC007462
• Symbole du gène: CKM
• Identification du gène (NCBI): 1158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42 kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody is specific to CKM.

Publications

Species	Application	Title
	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Cell Prolif; KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.; Authors - Jing Hao
mouse	WB	Toxicology; Pb inhibited C2C12 myoblast differentiation by regulating HDAC2; Authors - Xiaozhen Gu
Bacterial	WB	Mol Pharmacol; Naturally Occurring Mutations to Muscle-Type Creatine Kinase Impact Its Canonical and Pharmacological Activities in a Substrate-Dependent Manner In Vitro.; Authors - Eric P Mosher
mouse	WB	BMC Musculoskelet Disord; Ang-(1-7) protects skeletal muscle function in aged mice.; Authors - Ying Li