Anticorps Polyclonal de lapin anti-CKM-Specific

CKM-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 18712-1-AP

Synonymes

CKM, Creatine phosphokinase M-type, Creatine kinase M-type, CPK-M, CKMM



Applications testées

Résultats positifs en WBtissu cérébral de souris, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu d'intestin grêle de souris
Résultats positifs en IHCtissu de muscle squelettique de souris, tissu cardiaque de rat
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

18712-1-AP cible CKM-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, souris, Bactérie
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet creatine kinase, muscle
Masse moléculaire calculée 43 kDa
Poids moléculaire observé 43 kDa
Numéro d’acquisition GenBankBC007462
Symbole du gène CKM
Identification du gène (NCBI) 1158
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKM, also named CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in the heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain, and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB exists in the myocardium, and BB exists in many tissues, especially the brain. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme. This oxidized form of creatine kinase was rapidly reconverted to the 42 kDa species by the addition of reducing agents concomitant with restoration of activity. (PMID: 10827185). This antibody is specific to CKM.

Protocole

Product Specific Protocols
WB protocol for CKM-Specific antibody 18712-1-APDownload protocol
IHC protocol for CKM-Specific antibody 18712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
mouseWB

Cell Prolif

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Authors - Jing Hao
mouseWB

Toxicology

Pb inhibited C2C12 myoblast differentiation by regulating HDAC2

Authors - Xiaozhen Gu
BacterialWB

Mol Pharmacol

Naturally Occurring Mutations to Muscle-Type Creatine Kinase Impact Its Canonical and Pharmacological Activities in a Substrate-Dependent Manner In Vitro.

Authors - Eric P Mosher
mouseWB

BMC Musculoskelet Disord

Ang-(1-7) protects skeletal muscle function in aged mice.

Authors - Ying Li
mouse,ratWB,IF

Phytomedicine

Ligustilide covalently binds to Cys254 of the creatine kinase, M-type protein, ameliorating acute myocardial ischemia by enhancing the creatine phosphate level

Authors - Kaixue Zhang
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