Anticorps Monoclonal anti-CKM-Specific

CKM-Specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G3F6

N° de cat : 60177-1-PBS

Synonymes

CKM, 2G3F6, Creatine kinase M-type, CPK-M, CKMM

---

Galerie de données de validation

• 

  WB analysis of human skeletal muscle using 60177-1-Ig (same clone as 60177-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 60177-1-Ig (same clone as 60177-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 60177-1-Ig (same clone as 60177-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

60177-1-PBS cible CKM-Specific dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: creatine kinase, muscle
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa, 90 kDa, 130 kDa
• Numéro d’acquisition GenBank: BC007462
• Symbole du gène: CKM
• Identification du gène (NCBI): 1158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383).

{{ptg:RelatedPrimaryAntibodies}}