Anticorps Polyclonal de lapin anti-CKMT1A

• Phare
• Validé par KD/KO

CKMT1A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15346-1-PBS

Synonymes

Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi

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Galerie de données de validation

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  WB analysis of rat heart using 15346-1-AP (same clone as 15346-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15346-1-AP (same clone as 15346-1-PBS)

  WB result of CKMT1A antibody (15346-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CKMT1A transfected HEK-293 cells. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 cells using 15346-1-AP (same clone as 15346-1-PBS)

  A549 cells (shcontrol and shRNA of CKMT1A) were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:30. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 15346-1-AP (same clone as 15346-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 15346-1-AP (same clone as 15346-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 15346-1-AP (same clone as 15346-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 15346-1-AP (same clone as 15346-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 15346-1-AP (same clone as 15346-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 15346-1-AP (same clone as 15346-1-PBS)

  IP result of anti-CKMT1A (IP:15346-1-AP, 4ug; Detection:15346-1-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15346-1-AP (same clone as 15346-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15346-1-AP (same clone as 15346-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15346-1-PBS cible CKMT1A dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKMT1A Protéine recombinante Ag7583
• Nom complet: creatine kinase, mitochondrial 1A
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC001926
• Symbole du gène: CKMT1A
• Identification du gène (NCBI): 548596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

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