Anticorps Polyclonal de lapin anti-CLASP2

• Phare
• Validé par KD/KO

CLASP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 12942-1-AP

Synonymes

CLASP2, CLIP associating protein 2, hOrbit2, KIAA0627, Protein Orbit homolog 2

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Galerie de données de validation

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  WB analysis of human brain using 12942-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12942-1-AP (CLASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12942-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12942-1-AP (CLASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12942-1-AP (CLASP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12942-1-AP (CLASP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12942-1-AP

  Immunofluorescent analysis of HepG2 cells using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12942-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12942-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12942-1-AP cible CLASP2 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLASP2 Protéine recombinante Ag4012
• Nom complet: cytoplasmic linker associated protein 2
• Masse moléculaire calculée: 1294 aa, 141 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: BC029035
• Symbole du gène: CLASP2
• Identification du gène (NCBI): 23122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLASP2 (CLIP-associating protein 2) is a brain specific protein belongs to CLASP family. CLASP1 and CLASP2 regulate microtubule plus-end dynamics in brine cortex. CLASP2 is required for the orientation of stabilized microtubules toward the leading edge, It may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms. Phosphorylation of CLASP2 by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Publications

Species	Application	Title
mouse	WB	J Bone Miner Res; Regulation of bone resorption and sealing zone formation in osteoclasts occurs through protein kinase B-mediated microtubule stabilization.; Authors - Matsumoto Takumi T

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (07-06-2020)

30ug HEK protein loaded, and CLASP2 levels detected for control cells or cells transfected with CLASP2 siRNA

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK 293T

CLASP2 Antibody Western Blot validation (1:500 dilution) in HEK 293T (Cat no:12942-1-AP)