Anticorps Recombinant de lapin anti-CLCA1

CLCA1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240941G4

N° de cat : 83829-7-PBS

Synonymes

Calcium-activated chloride channel protein 1, Calcium-activated chloride channel family member 1, CaCC-1, CACC1, CACC

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Galerie de données de validation

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  WB analysis using 83829-7-RR (same clone as 83829-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83829-7-RR (CLCA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83829-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83829-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83829-7-RR against Human CLCA1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83829-7-PBS cible CLCA1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLCA1 Protéine recombinante Ag18176
• Nom complet: chloride channel accessory 1
• Masse moléculaire calculée: 914 aa, 100 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC156805
• Symbole du gène: CLCA1
• Identification du gène (NCBI): 1179
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)

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