Anticorps Recombinant de lapin anti-CLEC12A

CLEC12A Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

240791B7

N° de cat : CL594-98083

Synonymes

240791B7, C type lectin like molecule 1, CD371, CLL 1, CLL1

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using CL594-98083

  1x10^6 human peripheral blood leukocytes were surface stained with FITC Plus Anti-Human CD16, and 5 ul CoraLite®594 Anti-Human CLEC12A Rabbit RecAb (CL594-98083, Clone: 240791B7) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL594-98083

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite®594 Anti-Human CLEC12A Rabbit RecAb (CL594-98083, Clone: 240791B7) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98083 cible CLEC12A dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLEC12A Protéine recombinante Eg0024
• Nom complet: C-type lectin domain family 12, member A
• Masse moléculaire calculée: 31 kDa
• Numéro d’acquisition GenBank: BC126291
• Symbole du gène: CLEC12A
• Identification du gène (NCBI): 160364
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).

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