Anticorps Recombinant de lapin anti-CLEC14A

CLEC14A Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241206D3

N° de cat : 84327-1-PBS

Synonymes

241206D3, C14orf27, CEG1, C-type lectin domain family 14 member A, EGFR-5

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Galerie de données de validation

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  WB analysis using 84327-1-RR (same clone as 84327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84327-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84327-1-RR (same clone as 84327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84327-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84327-1-RR (same clone as 84327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84327-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84327-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

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Informations sur le produit

84327-1-PBS cible CLEC14A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLEC14A Protéine recombinante Ag21081
• Nom complet: C-type lectin domain family 14, member A
• Masse moléculaire calculée: 490 aa, 52 kDa
• Poids moléculaire observé: 52 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC031567
• Symbole du gène: CLEC14A
• Identification du gène (NCBI): 161198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

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