Anticorps Polyclonal de lapin anti-CLIC1

• Phare
• Validé par KD/KO

CLIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 14545-1-AP

Synonymes

G6, NCC27

---

Galerie de données de validation

• 

  WB analysis of HL-60 using 14545-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14545-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14545-1-AP

  IP result of anti-CLIC1 (IP:14545-1-AP, 4ug; Detection:14545-1-AP 1:300) with HeLa cells lysate 1200ug.

• 

  IHC staining of mouse colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14545-1-AP (CLIC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14545-1-AP (CLIC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14545-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14545-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CLIC1 antibody (14545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 14545-1-AP

  Immunofluorescent analysis of Hela cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de côlon de souris, tissu de côlon humain, tissu pancréatique humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

14545-1-AP cible CLIC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLIC1 Protéine recombinante Ag6049
• Nom complet: chloride intracellular channel 1
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-32 kDa
• Numéro d’acquisition GenBank: BC064527
• Symbole du gène: CLIC1
• Identification du gène (NCBI): 1192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).

Publications

Species	Application	Title
		Nat Commun; CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.; Authors - Tiantian Tang
mouse	WB	Alzheimers Res Ther; Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis; Authors - Deng-Pan Wu
rat	IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li
human	WB	Transl Oncol; Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling; Authors - Huiwu Geng ; KD Validated
		Cell Biochem Biophys; CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.; Authors - Dezhao Lu
human	IHC	Anticancer Res; Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.; Authors - Yuichiro Yasuda

{{ptg:RelatedPrimaryAntibodies}}