Anticorps Recombinant de lapin anti-CLIC1

CLIC1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250143E11

N° de cat : 85831-4-PBS

Synonymes

G6, NCC27

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Galerie de données de validation

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  WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 85831-4-RR (same clone as 85831-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85831-4-RR (same clone as 85831-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85831-4-RR (same clone as 85831-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLIC1 antibody (85831-4-RR, Clone: 250143E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85831-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85831-4-RR against Human CLIC1 were performed. The affinity constant is 22.0 nM.

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Informations sur le produit

85831-4-PBS cible CLIC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLIC1 Protéine recombinante Ag6049
• Nom complet: chloride intracellular channel 1
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-32 kDa
• Numéro d’acquisition GenBank: BC064527
• Symbole du gène: CLIC1
• Identification du gène (NCBI): 1192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).

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