Anticorps Recombinant de lapin anti-CLIC2

CLIC2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240726D1

N° de cat : 83730-3-RR

Synonymes

XAP121, 240726D1

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Galerie de données de validation

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  WB analysis of K-562 using 83730-3-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83730-3-RR (CLIC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83730-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83730-3-RR (CLIC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83730-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83730-3-RR against Human CLIC2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules K-562, tissu cardiaque humain, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83730-3-RR cible CLIC2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLIC2 Protéine recombinante Ag6766
• Nom complet: chloride intracellular channel 2
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC005367
• Symbole du gène: CLIC2
• Identification du gène (NCBI): 1193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLIC2 (chloride intracellular channel 2), also known as CLCNL2. It is expected to be located in cytoplasm and membrane. CLIC-2 exists as a monomer. It also has an unusual enzyme activity profile. While the CXXC active site motif is conserved between CLIC-2 and the glutaredoxins, no thiol transferase activity was detected. In contrast, low glutathione peroxidase activity was recorded. CLIC-2 was found to be widely distributed in tissues including heart and skeletal muscle. Functional studies showed that CLIC-2 inhibited cardiac ryanodine receptor Ca2+ release channels in lipid bilayers when added to the cytoplasmic side of the channels and inhibited Ca2+ release from cardiac sarcoplasmic reticulum vesicles. The calculated molecular weight of CLIC2 is 28 kDa.

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