Anticorps Recombinant de lapin anti-CLIC3

CLIC3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242326H7

N° de cat : 84759-4-PBS

Synonymes

242326H7

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Galerie de données de validation

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  WB analysis of JAR using 84759-4-RR (same clone as 84759-4-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84759-4-RR (same clone as 84759-4-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84759-4-RR (same clone as 84759-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84759-4-RR (CLIC3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84759-4-RR (same clone as 84759-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84759-4-RR (CLIC3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84759-4-RR (same clone as 84759-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLIC3 antibody (84759-4-RR, Clone: 242326H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84759-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84759-4-RR against Human CLIC3 were performed. The affinity constant is 0.634 nM.

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Informations sur le produit

84759-4-PBS cible CLIC3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLIC3 Protéine recombinante Ag8757
• Nom complet: chloride intracellular channel 3
• Masse moléculaire calculée: 236 aa, 26 kDa
• Poids moléculaire observé: 27-32 kDa
• Numéro d’acquisition GenBank: BC007012
• Symbole du gène: CLIC3
• Identification du gène (NCBI): 9022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.

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