Anticorps Recombinant de lapin anti-CLIC3

CLIC3 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242326H7

N° de cat : 84759-4-RR

Synonymes

242326H7

---

Galerie de données de validation

• 

  WB analysis of JAR using 84759-4-RR

  JAR cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 84759-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 84759-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84759-4-RR (CLIC3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84759-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84759-4-RR (CLIC3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 84759-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLIC3 antibody (84759-4-RR, Clone: 242326H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84759-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84759-4-RR against Human CLIC3 were performed. The affinity constant is 0.634 nM.

---

Applications testées

• Résultats positifs en WB: cellules JAR, cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84759-4-RR cible CLIC3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLIC3 Protéine recombinante Ag8757
• Nom complet: chloride intracellular channel 3
• Masse moléculaire calculée: 236 aa, 26 kDa
• Poids moléculaire observé: 27-32 kDa
• Numéro d’acquisition GenBank: BC007012
• Symbole du gène: CLIC3
• Identification du gène (NCBI): 9022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.

{{ptg:RelatedPrimaryAntibodies}}