Anticorps Polyclonal de lapin anti-CLK1

CLK1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20439-1-PBS

Synonymes

CDC-like kinase 1, CLK, EC:2.7.12.1, STY

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Galerie de données de validation

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  WB analysis using 20439-1-AP (same clone as 20439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20439-1-AP (same clone as 20439-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20439-1-AP (same clone as 20439-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 20439-1-AP (same clone as 20439-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 20439-1-AP (same clone as 20439-1-PBS)

  IP result of anti-CLK1 (IP:20439-1-AP, 4ug; Detection:20439-1-AP 1:300) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20439-1-PBS cible CLK1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLK1 Protéine recombinante Ag14214
• Nom complet: CDC-like kinase 1
• Masse moléculaire calculée: 484 aa, 57 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC031549
• Symbole du gène: CLK1
• Identification du gène (NCBI): 1195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CLK1 (CDC-like kinase 1) is the dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. It phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. It can phosphorylate: SRSF1, SRSF3 and PTPN1 (PMID: 10480872; 19168442). It also regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (PMID: 19168442). Elevated CLK1 activity is frequently detected in cancers, where it alters splice isoform balance to promote proliferation, invasion and chemoresistance, making it an emerging therapeutic target.

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