Anticorps Recombinant de lapin anti-CLOCK

CLOCK Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1I23

N° de cat : 82829-1-PBS

Synonymes

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

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Galerie de données de validation

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  WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82829-1-RR (same clone as 82829-1-PBS)

  WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82829-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82829-1-RR against Human CLOCK were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82829-1-PBS cible CLOCK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLOCK Protéine recombinante Ag30011
• Nom complet: clock homolog (mouse)
• Masse moléculaire calculée: 846 aa, 95 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC041878
• Symbole du gène: CLOCK
• Identification du gène (NCBI): 9575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

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