Anticorps Recombinant de lapin anti-CLOCK

• Phare
• Validé par KD/KO

CLOCK Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1I23

N° de cat : 82829-1-RR

Synonymes

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

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Galerie de données de validation

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  WB analysis using 82829-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82829-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82829-1-RR

  WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells.

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  IHC staining of mouse liver using 82829-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 82829-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82829-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82829-1-RR against Human CLOCK were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules NIH/3T3, cellules PC-3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

82829-1-RR cible CLOCK dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLOCK Protéine recombinante Ag30011
• Nom complet: clock homolog (mouse)
• Masse moléculaire calculée: 846 aa, 95 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC041878
• Symbole du gène: CLOCK
• Identification du gène (NCBI): 9575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

Publications

Species	Application	Title
rat	WB	Sci Rep; Involvement of circadian clock protein PER2 in controlling sleep deprivation induced HMGB1 up-regulation by targeting p300 in the cortex; Authors - Min Zhang

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