Anticorps Polyclonal de lapin anti-CLPB

CLPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 15743-1-AP

Synonymes

SKD3, Mitochondrial disaggregase, cleaved form, Mitochondrial disaggregase, HSP78, EC:3.6.1.-

---

Galerie de données de validation

• 

  WB analysis using 15743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15743-1-AP (CLPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15743-1-AP (CLPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15743-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15743-1-AP (CLPB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 15743-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15743-1-AP (CLPB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 15743-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLPB antibody (15743-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

15743-1-AP cible CLPB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLPB Protéine recombinante Ag8165
• Nom complet: ClpB caseinolytic peptidase B homolog (E. coli)
• Masse moléculaire calculée: 707 aa, 79 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC006404
• Symbole du gène: CLPB
• Identification du gène (NCBI): 81570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLPB encodes a protein of 707 aa with a predicted molecular mass of approximately 79 kDa, presenting two conserved ATP-binding domains. CLPB is a member of the AAA+ protein family. This potential internal translation initiation site suggests that a smaller form of ClpB, with a theoretical molecular mass of approximately 58 kDa, may be produced. (PMID: 25597510, PMID: 255957260)

Publications

Species	Application	Title
	WB	Sci Adv; Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding; Authors - Arpit Gupta
human	WB	Elife; Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations.; Authors - Ryan R Cupo
human	WB	iScience; Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region.; Authors - Takahiro Yoshinaka
human	WB	Biochim Biophys Acta Mol Cell Res; Impact of PARL-mediated mitochondrial protease activity on calcium regulation; Authors - Donato D'Angelo
mouse	WB	Elife; Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis; Authors - Enrico Radaelli
human,rat	WB,IHC	Int J Mol Sci; Exploring Potential Diagnostic Biomarkers for Mechanical Asphyxia in the Heart Based on Proteomics Technology; Authors - Yuebing Huang

{{ptg:RelatedPrimaryAntibodies}}