Anticorps Recombinant de lapin anti-CLPS

CLPS Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241565H3

N° de cat : 84224-2-PBS

Synonymes

Colipase, 241565H3

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Galerie de données de validation

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  WB analysis of rat pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (84224-2-RR, Clone: 241565H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01160-1

  Cytometric bead array standard curve of MP01160-1, CLPS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84224-1-PBS. Detection antibody: 84224-2-PBS. Standard: Ag2321. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84224-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84224-2-PBS cible CLPS dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLPS Protéine recombinante Ag2321
• Nom complet: colipase, pancreatic
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC017897
• Symbole du gène: CLPS
• Identification du gène (NCBI): 1208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

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