Anticorps Recombinant de lapin anti-CLPX

• Phare
• Validé par KD/KO

CLPX Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230346H1

N° de cat : 83097-1-PBS

Synonymes

230346H1, EPP2

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Galerie de données de validation

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  WB analysis of A549 using 83097-1-RR (same clone as 83097-1-PBS)

  WB result of CLPX antibody (83097-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  WB analysis using 83097-1-RR (same clone as 83097-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83097-1-RR (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00277-3

  The mean CLPX concentration was determined to be 163.0 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 80.9 ng/mL in A549 cell extract based on a 1.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00277-3

  Sandwich ELISA standard curve of MP00277-3, Human CLPX Recombinant Matched Antibody Pair - PBS only. 83097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34213. 83097-1-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL.

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  IHC staining of human liver using 83097-1-RR (same clone as 83097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 83097-1-RR (same clone as 83097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 83097-1-RR (same clone as 83097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 83097-1-RR (same clone as 83097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83097-1-RR (same clone as 83097-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CLPX antibody (83097-1-RR, Clone: 230346H1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83097-1-RR (same clone as 83097-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human CLPX (83097-1-RR, Clone:230346H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83097-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83097-1-RR against Human CLPX were performed. The affinity constant is 2.13 nM.

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Informations sur le produit

83097-1-PBS cible CLPX dans les applications de WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLPX Protéine recombinante Ag34213
• Nom complet: ClpX caseinolytic peptidase X homolog (E. coli)
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: NM_006660
• Symbole du gène: CLPX
• Identification du gène (NCBI): 10845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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