Anticorps Polyclonal de lapin anti-Calsyntenin-1

Calsyntenin-1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 12788-1-AP

Synonymes

ALC ALPHA, Alcadein alpha, alcalpha1, alcalpha2, C terminal fragment 1 alpha, calsyntenin 1, Calsyntenin-1, CLSTN1, CS1, CSTN1, KIAA0911, PIK3CD, SAlc alpha CTF1 alpha, XB31alpha

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Galerie de données de validation

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  WB analysis of human brain using 12788-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12788-1-AP

  IP Result of anti-CLSTN1 (IP:12788-1-AP, 4ug; Detection:12788-1-AP 1:300) with mouse brain tissue lysate 5600ug.

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  IHC staining of mouse brain using 12788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12788-1-AP cible Calsyntenin-1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calsyntenin-1 Protéine recombinante Ag3504
• Nom complet: calsyntenin 1
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 109-130 kDa
• Numéro d’acquisition GenBank: BC033902
• Symbole du gène: CLSTN1
• Identification du gène (NCBI): 22883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).

Publications

Species	Application	Title
mouse	IF	J Neurosci; The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites.; Authors - Muresan Virgil V
human	WB	Front Oncol; Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.; Authors - Yi-Yuan Sun
rat	WB,IHC	Cardiovasc Drugs Ther; Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats; Authors - Mingxiang Zhu
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
human	WB	Cell Death Discov; ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer; Authors - Chengguo Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (12-09-2019)

This antibody gave a clean band around 100 kD, like that is shown in its datasheet. However, in literature, people showed that this protein runs as doublet bands at 130 - 150 kD due to its glycosylation. Therefore, this antibody is highly suspicious for recognizing another protein rather than Clstn1 itself.

• Applications: Western Blot,