Anticorps Monoclonal anti-CLTB

CLTB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9C2

N° de cat : 66270-1-PBS

Synonymes

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

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Galerie de données de validation

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  WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66270-1-PBS cible CLTB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CLTB Protéine recombinante Ag24347
• Nom complet: clathrin, light chain (Lcb)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 32-38 kDa
• Numéro d’acquisition GenBank: BC006457
• Symbole du gène: CLTB
• Identification du gène (NCBI): 1212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

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