Anticorps Polyclonal de lapin anti-CLUAP1

• Phare
• Validé par KD/KO

CLUAP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 17470-1-AP

Synonymes

Qilin, KIAA0643, Clusterin-associated protein 1, clusterin associated protein 1

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Galerie de données de validation

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  WB analysis of mouse testis using 17470-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 17470-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17470-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17470-1-AP

  IP result of anti-CLUAP1 (IP:17470-1-AP, 4ug; Detection:17470-1-AP 1:600) with HepG2 cells lysate 2400ug.

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  IF Staining of MDCK using 17470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CLUAP1 antibody (17470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules COLO 320, cellules HepG2, cellules Y79
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF/ICC: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

17470-1-AP cible CLUAP1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLUAP1 Protéine recombinante Ag11419
• Nom complet: clusterin associated protein 1
• Masse moléculaire calculée: 413 aa, 48 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC017070
• Symbole du gène: CLUAP1
• Identification du gène (NCBI): 23059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).

Publications

Species	Application	Title
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	WB,IF	Mol Cell Proteomics; CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.; Authors - Tina Beyer ; KO Validated
human	WB	Hum Mol Genet; Defective IFT57 underlies a novel cause of Bardet-Biedl syndrome; Authors - Alexandra Nitoiu
human	IF	Elife; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie

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