Anticorps Polyclonal de lapin anti-CLYBL

CLYBL Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17314-1-AP

Synonymes

citrate lyase beta like, CLB, CLYBL

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Galerie de données de validation

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  WB analysis using 17314-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17314-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17314-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17314-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17314-1-AP (CLYBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17314-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17314-1-AP (CLYBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules THP-1, tissu cardiaque humain
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

17314-1-AP cible CLYBL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLYBL Protéine recombinante Ag11283
• Nom complet: citrate lyase beta like
• Masse moléculaire calculée: 340 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC034360
• Symbole du gène: CLYBL
• Identification du gène (NCBI): 171425
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLYBL, also named as CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There're two isoforms with MW 31-33 kDa and 35-37 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Positive feedback loop involving AMPK and CLYBL acetylation links metabolic rewiring and inflammatory responses; Authors - Wenke Wang
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
human	WB,IHC	J Transl Med; Acetylation suppresses breast cancer progression by sustaining CLYBL stability; Authors - Xinyue Deng

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