Anticorps Recombinant de lapin anti-CLYBL

CLYBL Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242785F10

N° de cat : 85399-4-PBS

Synonymes

(3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like

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Galerie de données de validation

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  WB analysis using 85399-4-RR (same clone as 85399-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 85399-4-RR (same clone as 85399-4-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85399-4-RR (same clone as 85399-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85399-4-RR (CLYBL antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85399-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85399-4-RR against Human CLYBL were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85399-4-PBS cible CLYBL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CLYBL Protéine recombinante Ag11283
• Nom complet: citrate lyase beta like
• Masse moléculaire calculée: 340 aa, 37 kDa
• Poids moléculaire observé: 30-40 kDa
• Numéro d’acquisition GenBank: BC034360
• Symbole du gène: CLYBL
• Identification du gène (NCBI): 171425
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CLYBL, also named CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating the conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There are two isoforms with MW 31-33 kDa and 35-37 kDa.

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