Anticorps Polyclonal de lapin anti-CMIP

• Phare
• Validé par KD/KO

CMIP Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 12851-1-AP

Synonymes

c Maf inducing protein, c Mip, CMIP, KIAA1694, Tc Mip, TCMIP

---

Galerie de données de validation

• 

  WB analysis of K-562 using 12851-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 12851-1-AP

  IP Result of anti-CMIP (IP:12851-1-AP, 4ug; Detection:12851-1-AP 1:300) with Raji cells lysate 2000ug.

• 

  IHC staining of human gliomas using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules Raji
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer de l'estomac humain, tissu de cancer du poumon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12851-1-AP cible CMIP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CMIP Protéine recombinante Ag3475
• Nom complet: c-Maf-inducing protein
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC038113
• Symbole du gène: CMIP
• Identification du gène (NCBI): 80790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.

Publications

Species	Application	Title
mouse	WB,IF	Cell Signal; Inhibition of nephrin activation by c-mip through Csk-Cbp-Fyn axis plays a critical role in Angiotensin II-induced podocyte damage.; Authors - Yu Lixia L ; KD Validated
mouse	WB	Cell Physiol Biochem; Induction of C-Mip by IL-17 Plays an Important Role in Adriamycin-Induced Podocyte Damage.; Authors - Yanbo Liu
human	WB,IHC	Biomed Res Int; CMIP Promotes Proliferation and Metastasis in Human Glioma.; Authors - Bin Wang ; KD Validated
human	WB,IHC	Pathol Res Pract; PMID: 31822364; Authors - Ru Xiang ; KD Validated
		Mol Med Rep; c‑Maf inducing protein inhibits cofilin‑1 activity and alters podocyte cytoskeleton organization.; Authors - Lixia Yu
human	WB,IHC	Mol Med Rep; CMIP is oncogenic in human gastric cancer cells.; Authors - Jianlin Zhang