Anticorps Polyclonal de lapin anti-UMP/CMP kinase

UMP/CMP kinase Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11360-1-AP

Synonymes

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

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Galerie de données de validation

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  WB analysis of mouse pancreas using 11360-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11360-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11360-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11360-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11360-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu de muscle squelettique humain, tissu pancréatique humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11360-1-AP cible UMP/CMP kinase dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UMP/CMP kinase Protéine recombinante Ag1920
• Nom complet: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Masse moléculaire calculée: 228 aa, 26 kDa
• Poids moléculaire observé: 22-26 kDa
• Numéro d’acquisition GenBank: BC014961
• Symbole du gène: CMPK1
• Identification du gène (NCBI): 51727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

Publications

Species	Application	Title
		J Proteomics; Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.; Authors - Víctor Llombart