Anticorps Recombinant de lapin anti-UMP/CMP kinase

UMP/CMP kinase Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251233D3

N° de cat : 86475-1-RR

Synonymes

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

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Galerie de données de validation

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  WB analysis using 86475-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86475-1-RR (CMPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 86475-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 86475-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 86475-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CMPK1 antibody (86475-1-RR, Clone: 251233D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86475-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86475-1-RR against Human UMP/CMP kinase were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HL-60, cellules HT-29, cellules U-251
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86475-1-RR cible UMP/CMP kinase dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UMP/CMP kinase Protéine recombinante Ag1920
• Nom complet: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Masse moléculaire calculée: 228 aa, 26 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC014961
• Symbole du gène: CMPK1
• Identification du gène (NCBI): 51727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

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