Anticorps Monoclonal anti-CNBP

• Phare
• Validé par KD/KO

CNBP Monoclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1H1D12

Avis (1)

N° de cat : 67109-1-Ig

Synonymes

CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9

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Galerie de données de validation

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  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67109-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67109-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67109-1-Ig

  IP result of anti-CNBP (IP:67109-1-Ig, 5ug; Detection:67109-1-Ig 1:500) with HeLa cells lysate 1600 ug.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules A549, cellules Caco-2, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HT-29, cellules Jurkat, cellules K-562, cellules NCI-H1299, cellules Neuro-2a, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

67109-1-Ig cible CNBP dans les applications de WB, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNBP Protéine recombinante Ag7114
• Nom complet: CCHC-type zinc finger, nucleic acid binding protein
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19-25 kDa
• Numéro d’acquisition GenBank: BC000288
• Symbole du gène: CNBP
• Identification du gène (NCBI): 7555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP,WB	Nat Microbiol; The SARS-CoV-2 RNA-protein interactome in infected human cells.; Authors - Nora Schmidt ; KO Validated
human	WB,IP	PLoS One; CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA; Authors - Yunlu Liu
mouse	WB	J Mol Cell Cardiol; Cellular nucleic acid binding protein facilitates cardiac repair after myocardial infarction by activating β-catenin signaling; Authors - Chong Du
human	WB	PLoS One; circTADA2A inhibited SLC38A1 expression and suppresses melanoma progression through the prevention of CNBP trans-activation; Authors - Longjun Zhang
human,mouse	WB,IP,IF	bioRxiv; A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions; Authors - Konstadinos Moissoglu ; KO Validated
human,mouse	WB,RIP,IF	Cell Rep; A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions; Authors - Konstadinos Moissoglu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katherine (Verified Customer) (09-25-2023)

The band was clear

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T

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