Anticorps Polyclonal de lapin anti-CNDP1

CNDP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 17759-1-AP

Synonymes

CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 17759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17759-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 17759-1-AP

  Immunofluorescent analysis of A431 cells using 17759-1-AP (CNDP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

17759-1-AP cible CNDP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNDP1 Protéine recombinante Ag12127
• Nom complet: carnosine dipeptidase 1 (metallopeptidase M20 family)
• Masse moléculaire calculée: 508 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC110295
• Symbole du gène: CNDP1
• Identification du gène (NCBI): 84735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-Ala-His dipeptidase (CNDP1) encodes the secreted serum carnosinase, which is synthesized in the brain and secreted into the systematic circulation, CNDP1 hydrolyzes L-carnosine and is essential for glucose metabolism and the transportation of activated fatty acids across the mitochondrial membrane (PMID: 32111749, 16046297).

Publications

Species	Application	Title
human	IHC	Amino Acids; Serum and urinary carnosinase-1 correlate with kidney function and inflammation; Authors - Shiqi Zhang

{{ptg:RelatedPrimaryAntibodies}}