Anticorps Recombinant de lapin anti-CNDP2

CNDP2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242373E4

N° de cat : 84924-7-RR

Synonymes

242373E4, CNDP dipeptidase 2, CPGL, Cytosolic non-specific dipeptidase, EC:3.4.13.18

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Galerie de données de validation

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  WB analysis using 84924-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84924-7-RR (CNDP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat stomach using 84924-7-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84924-7-RR (CNDP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 84924-7-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84924-7-RR (CNDP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84924-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84924-7-RR against Human CNDP2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules U2OS, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu d'estomac de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84924-7-RR cible CNDP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CNDP2 Protéine recombinante Ag6684
• Nom complet: CNDP dipeptidase 2 (metallopeptidase M20 family)
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 44-53 kDa
• Numéro d’acquisition GenBank: BC001375
• Symbole du gène: CNDP2
• Identification du gène (NCBI): 55748
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa.
The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).

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