Anticorps Monoclonal anti-CNKSR1

CNKSR1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1E11H8

N° de cat : 66796-1-Ig

Synonymes

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

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Galerie de données de validation

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  WB analysis of MCF-7 using 66796-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66796-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66796-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66796-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules Caco-2, cellules HEK-293, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66796-1-Ig cible CNKSR1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNKSR1 Protéine recombinante Ag27855
• Nom complet: connector enhancer of kinase suppressor of Ras 1
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC011604
• Symbole du gène: CNKSR1
• Identification du gène (NCBI): 10256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.

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