Anticorps Recombinant de lapin anti-CNKSR2

CNKSR2 Recombinant Antibody for Indirect ELISA, Cytometric bead array, WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

Indirect ELISA, Cytometric bead array, WB

Conjugaison

Non conjugué

CloneNo.

240350D6

N° de cat : 83354-3-PBS

Synonymes

KSR2, KIAA0902, Connector enhancer of KSR 2, CNK2, CNK homolog protein 2

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Galerie de données de validation

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  WB analysis using 83354-3-RR (same clone as 83354-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83354-3-PBS in a different storage buffer formulation.

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  WB analysis using 83354-3-RR (same clone as 83354-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83354-3-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 83354-3-RR (same clone as 83354-3-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CNKSR2 Recombinant antibody (83354-3-RR, Clone:240350D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83354-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00389-1

  Cytometric bead array standard curve of MP00389-1, CNKSR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83354-2-PBS. Detection antibody: 83354-3-PBS. Standard: Ag21174. Range: 3.125-100ng/mL.

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  Affinity and Kinetic Characterization of 83354-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83354-3-RR against Human CNKSR2 were performed. The affinity constant is 11.1 nM.

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Informations sur le produit

83354-3-PBS cible CNKSR2 dans les applications de Indirect ELISA, Cytometric bead array, WB et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CNKSR2 Protéine recombinante Ag21174
• Nom complet: connector enhancer of kinase suppressor of Ras 2
• Masse moléculaire calculée: 1034 aa, 118 kDa
• Numéro d’acquisition GenBank: BC126121
• Symbole du gène: CNKSR2
• Identification du gène (NCBI): 22866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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