Anticorps Recombinant de lapin anti-CNKSR2

CNKSR2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240350D6

N° de cat : 83354-3-RR

Synonymes

KSR2, KIAA0902, Connector enhancer of KSR 2, CNK2, CNK homolog protein 2

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Galerie de données de validation

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  WB analysis using 83354-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83354-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 83354-3-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CNKSR2 Recombinant antibody (83354-3-RR, Clone:240350D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83354-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83354-3-RR against Human CNKSR2 were performed. The affinity constant is 11.1 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules MDA-MB-231, cellules U-251, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83354-3-RR cible CNKSR2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CNKSR2 Protéine recombinante Ag21174
• Nom complet: connector enhancer of kinase suppressor of Ras 2
• Masse moléculaire calculée: 1034 aa, 118 kDa
• Poids moléculaire observé: 118 kDa, 102 kDa
• Numéro d’acquisition GenBank: BC126121
• Symbole du gène: CNKSR2
• Identification du gène (NCBI): 22866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNKSR2 is a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling.

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