Anticorps Monoclonal anti-CNN2

• Phare
• Validé par KD/KO

CNN2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G10F5

N° de cat : 66244-1-Ig

Synonymes

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

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Galerie de données de validation

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  WB analysis using 66244-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 66244-1-Ig

  WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

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  WB analysis of pig stomach using 66244-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 66244-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66244-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66244-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 66244-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 66244-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa, cellules HT-1080, cellules Jurkat, cellules K-562, tissu d'estomac de porc
• Résultats positifs en IHC: tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66244-1-Ig cible CNN2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNN2 Protéine recombinante Ag23594
• Nom complet: calponin 2
• Masse moléculaire calculée: 309 aa, 34 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC141818
• Symbole du gène: CNN2
• Identification du gène (NCBI): 1265
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.