Anticorps Monoclonal anti-CNN2

• Phare
• Validé par KD/KO

CNN2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G10F5

N° de cat : 66244-1-PBS

Synonymes

1G10F5, calponin 2, calponin2, Calponin-2, CNN 2

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Galerie de données de validation

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  WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

  WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis using 66244-1-Ig (same clone as 66244-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis of pig stomach using 66244-1-Ig (same clone as 66244-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 66244-1-Ig (same clone as 66244-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66244-1-Ig (same clone as 66244-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66244-1-PBS cible CNN2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNN2 Protéine recombinante Ag23594
• Nom complet: calponin 2
• Masse moléculaire calculée: 309 aa, 34 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC141818
• Symbole du gène: CNN2
• Identification du gène (NCBI): 1265
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

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