Anticorps Recombinant de lapin anti-CNN3

CNN3 Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251475E9

N° de cat : 86550-3-PBS

Synonymes

Calponin 3, Calponin3, Calponin-3, CNN 3

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Galerie de données de validation

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  WB analysis using 86550-3-RR (same clone as 86550-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86550-3-RR (CNN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86550-3-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 86550-3-RR (same clone as 86550-3-PBS)

  IP result of anti-CNN3 (IP:86550-3-RR, 4ug; Detection:86550-3-RR 1:2000) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86550-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86550-3-RR (same clone as 86550-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CNN3 antibody (86550-3-RR, Clone: 251475E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86550-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86550-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86550-3-RR against Human CNN3 were performed. The affinity constant is 1.83 nM.

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Informations sur le produit

86550-3-PBS cible CNN3 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CNN3 Protéine recombinante Ag2068
• Nom complet: calponin 3, acidic
• Masse moléculaire calculée: 329 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC025372
• Symbole du gène: CNN3
• Identification du gène (NCBI): 1266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain. CNN3 is the thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction.

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