Anticorps Monoclonal anti-CNOT1

• Phare
• Validé par KD/KO

CNOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1A10A11

Avis (2)

N° de cat : 66507-1-Ig

Synonymes

1A10A11, AD 005, AD-005, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 1

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Galerie de données de validation

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  WB analysis of HeLa using 66507-1-Ig

  WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells.

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  WB analysis using 66507-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66507-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66507-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 66507-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 66507-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 66507-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66507-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 66507-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 66507-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, tissu de cervelet humain, tissu rénal de porc, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

66507-1-Ig cible CNOT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNOT1 Protéine recombinante Ag5623
• Nom complet: CCR4-NOT transcription complex, subunit 1
• Masse moléculaire calculée: 267 kDa
• Poids moléculaire observé: 240-250 kDa
• Numéro d’acquisition GenBank: BC040523
• Symbole du gène: CNOT1
• Identification du gène (NCBI): 23019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.; Authors - Aishe A Sarshad ; KD Validated
human	WB	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
mouse	WB,IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (11-01-2024)

It was alright, seems to be good for hrp but not the best with flourescence

• Applications: Western Blot

FH

Roy (Verified Customer) (06-12-2024)

Great antibody to detect CNOT1 by WB from HeLa nuclear and cytoplasmic extracts (1/1000 - Overnight incubation at 4°C). Does also detect the cytoplasmic CNOT1 by immunofluorescence (1/250) in PFA 4% fixed HeLa cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000 (WB) - 1/250 (IF)
• Cell Tissue Type: HeLa

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