Anticorps Monoclonal anti-CNOT1

• Phare
• Validé par KD/KO

CNOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A10A11

N° de cat : 66507-1-PBS

Synonymes

1A10A11, AD 005, AD-005, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 1

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Galerie de données de validation

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  WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

  WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66507-1-Ig (same clone as 66507-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis of human cerebellum using 66507-1-Ig (same clone as 66507-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 66507-1-Ig (same clone as 66507-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 66507-1-Ig (same clone as 66507-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66507-1-PBS cible CNOT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNOT1 Protéine recombinante Ag5623
• Nom complet: CCR4-NOT transcription complex, subunit 1
• Masse moléculaire calculée: 267 kDa
• Poids moléculaire observé: 240-250 kDa
• Numéro d’acquisition GenBank: BC040523
• Symbole du gène: CNOT1
• Identification du gène (NCBI): 23019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

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