Anticorps Polyclonal de lapin anti-CNOT2

• Phare
• Validé par KD/KO

CNOT2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10313-1-AP

Synonymes

CCR4 associated factor 2, CDC36, CNOT2, HSPC131, MSTP046, NOT2, NOT2H

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Galerie de données de validation

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  WB analysis using 10313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10313-1-AP (CNOT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 10313-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10313-1-AP (CNOT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below

Informations sur le produit

10313-1-AP cible CNOT2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNOT2 Protéine recombinante Ag0347
• Nom complet: CCR4-NOT transcription complex, subunit 2
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC002597
• Symbole du gène: CNOT2
• Identification du gène (NCBI): 4848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ccr4-Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).

Publications

Species	Application	Title
human,mouse	WB	Stem Cells; Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.; Authors - Zheng Xiaofeng X ; KD Validated
mouse	WB	Stem Cell Reports; CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.; Authors - Xiaofeng Zheng
mouse	WB	RNA Biol; Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.; Authors - Dina Mostafa
mouse	WB	Stem Cells Dev; Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.; Authors - Kamon Masayoshi M ; KD Validated
mouse	WB	bioRxiv; Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance; Authors - Qing Chen
human	WB	Cells; Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability; Authors - Nafiseh Chalabi Hagkarim

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ayse (Verified Customer) (06-08-2022)

Used for IF experiment (dilution 1/100) and WB experiment (1/2000). The antibody works well for the 2 kind of experiment. In few weeks, I will also test for IP.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: HEK293T

CNOT2 Antibody Western Blot,Immunofluorescence validation (1/100 dilution) in HEK293T (Cat no:10313-1-AP)