Anticorps Polyclonal de lapin anti-CNOT6

• Phare
• Validé par KD/KO

CNOT6 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, RIP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 17935-1-AP

Synonymes

CCR4, CNOT6, Cytoplasmic deadenylase, KIAA1194

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Galerie de données de validation

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  WB analysis of Jurkat using 17935-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17935-1-AP (CNOT6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

17935-1-AP cible CNOT6 dans les applications de WB, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNOT6 Protéine recombinante Ag11506
• Nom complet: CCR4-NOT transcription complex, subunit 6
• Masse moléculaire calculée: 557 aa, 63.3 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC027476
• Symbole du gène: CNOT6
• Identification du gène (NCBI): 57472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells. This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (PMID: 12882519). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (PMID: 19558367). In addition, the subunit composition of this complex has been shown to vary among different tissues (PMID: 21741365). The various CNOT proteins display different functions, with CNOT6, CNOT6L, CNOT7, and CNOT8 exhibiting 3'-5' RNAse activity (PMID: 11889047). Research studies indicate that the CCR4-NOT deadenylase subunits CNOT6 and CNOT6L help mediate cell survival and play a role in cell proliferation, P-body formation, and regulation of gene expression (PMID: 21233283). Additional research evidence suggests that CNOT6 can act as a nuclear hormone receptor coactivator that associates with ZNF335 (NIF-1) to regulate expression from specific RARα regulated genes (PMID: 18180299).

Publications

Species	Application	Title
mouse	WB	Cell Res; Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis.; Authors - Lan-Tao Gou ; KD Validated
human	RIP	Cell; YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies; Authors - Zhenhua Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

Does work well to detect CNOT6 in HeLa cells on WB (1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

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