Anticorps Polyclonal de lapin anti-Cannabinoid receptor 1

Cannabinoid receptor 1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 17978-1-AP

Synonymes

CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1

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Galerie de données de validation

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  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17978-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cannabinoid receptor 1 antibody (17978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 17978-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cannabinoid receptor 1 (17978-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,
• Résultats positifs en cytométrie: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

17978-1-AP cible Cannabinoid receptor 1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cannabinoid receptor 1 Protéine recombinante Ag12371
• Nom complet: cannabinoid receptor 1 (brain)
• Masse moléculaire calculée: 472 aa, 53 kDa
• Poids moléculaire observé: 45 kDa, 66 kDa
• Numéro d’acquisition GenBank: BC074812
• Symbole du gène: CNR1
• Identification du gène (NCBI): 1268
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Publications

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
mouse	WB	Chin Med; Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis; Authors - Jingze Yang
mouse	WB	Hum Mol Genet; BDNF overexpression prevents cognitive deficit elicited by adolescent cannabis exposure and host susceptibility interaction.; Authors - Hadar Segal-Gavish
rat	IHC	Phytomedicine; Endocannabinoid signalling/cannabinoid receptor 2 is involved in icariin-mediated protective effects against bleomycin-induced pulmonary fibrosis.; Authors - Wenjing Du
rat	WB	Food Funct; L-Theanine regulates glutamine metabolism and immune function by binding to cannabinoid receptor 1.; Authors - An Liu
mouse,rat	WB,IHC,IF	Int J Cardiol; Indoxyl sulfate upregulates the cannabinoid type 1 receptor gene via an ATF3/c-Jun complex-mediated signaling pathway in the model of uremic cardiomyopathy.; Authors - Yu-Juei Hsu

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