Anticorps Polyclonal de lapin anti-CNRIP1

• Phare
• Validé par KD/KO

CNRIP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 16827-1-AP

Synonymes

C2orf32, CNRIP1, CRIP 1, CRIP1

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Galerie de données de validation

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  WB analysis of mouse brain using 16827-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16827-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 16827-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16827-1-AP (CNRIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16827-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16827-1-AP (CNRIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

16827-1-AP cible CNRIP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNRIP1 Protéine recombinante Ag10428
• Nom complet: cannabinoid receptor interacting protein 1
• Masse moléculaire calculée: 164 aa, 19 kDa
• Poids moléculaire observé: 13-25 kDa
• Numéro d’acquisition GenBank: BC011535
• Symbole du gène: CNRIP1
• Identification du gène (NCBI): 25927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNRIP1, also named as C2orf32, has two isoforms with MW 13-25 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC	Hepatology; DNA Methylation of Cannabinoid Receptor Interacting Protein 1 Promotes Pathogenesis of Intrahepatic Cholangiocarcinoma Through Suppressing Parkin-Dependent Pyruvate Kinase M2 Ubiquitination.; Authors - Diyu Chen
mouse	WB,IHC	Brain Res Bull; Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice.; Authors - Fu Yang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sandra (Verified Customer) (01-31-2025)

Western blot of purified His-tagged CNRIP1a protein dilutions. Li-Cor Odyssey using Li-Cor secondary donkey-anti rabbit IgG 800

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Purified CNRIP1a His-tagged protein from E-coli

CNRIP1 Antibody Western Blot validation (1:1000 dilution) in Purified CNRIP1a His-tagged protein from E-coli (Cat no:16827-1-AP)

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