Anticorps Polyclonal de lapin anti-CNTFR

CNTFR Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10796-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10796-1-AP (same clone as 10796-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 10796-1-AP (same clone as 10796-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 10796-1-AP (same clone as 10796-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10796-1-AP (same clone as 10796-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 10796-1-AP (same clone as 10796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 10796-1-AP (same clone as 10796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10796-1-PBS cible CNTFR dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNTFR Protéine recombinante Ag1189
• Nom complet: ciliary neurotrophic factor receptor
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC001492
• Symbole du gène: CNTFR
• Identification du gène (NCBI): 1271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.

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