Anticorps Monoclonal anti-COG1

COG1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D8D11

N° de cat : 68642-1-PBS

Synonymes

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

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Galerie de données de validation

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  WB analysis using 68642-1-Ig (same clone as 68642-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68642-1-Ig (same clone as 68642-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51262-1

  Cytometric bead array standard curve of MP51262-1, COG1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68642-1-PBS. Detection antibody: 68642-2-PBS. Standard:Ag33924. Range: 3.125-200 ng/mL.

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Informations sur le produit

68642-1-PBS cible COG1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin

• Réactivité: Humain, Lapin
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COG1 Protéine recombinante Ag33924
• Nom complet: component of oligomeric golgi complex 1
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 100-109 kDa
• Numéro d’acquisition GenBank: BC021985
• Symbole du gène: COG1
• Identification du gène (NCBI): 9382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).

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