Anticorps Monoclonal anti-COIL

COIL Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2G12B8

N° de cat : 67218-1-PBS

Synonymes

Coilin, 2G12B8, CLN80, p80 coilin, p80-coilin

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Galerie de données de validation

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  WB analysis of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67218-1-Ig (same clone as 67218-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67218-1-Ig (same clone as 67218-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COIL (67218-1-Ig, Clone:2G12B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67218-1-PBS cible COIL dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COIL Protéine recombinante Ag17171
• Nom complet: coilin
• Masse moléculaire calculée: 576 aa, 63 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC010385
• Symbole du gène: COIL
• Identification du gène (NCBI): 8161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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