Anticorps Polyclonal de lapin anti-Collagen Type X

Collagen Type X Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 26984-1-AP

Synonymes

COL10, COL10A1, Col10a 1, Collagen alpha-1(X) chain, Collagen type X alpha 1

---

Galerie de données de validation

• 

  WB analysis using 26984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 26984-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HCT 116, cellules ROS1728

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

26984-1-AP cible Collagen Type X dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type X Protéine recombinante Ag25741
• Nom complet: collagen, type X, alpha 1
• Masse moléculaire calculée: 680 aa, 66 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC130621
• Symbole du gène: COL10A1
• Identification du gène (NCBI): 1300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Collagen type X alpha 1 (COL10A1), a secreted, short-chain collagen, belongs to the collagen family, which is a major interstitial matrix component specifically expressed by hypertrophic chondrocytes. COL10A1 expression is elevated in many solid tumor types, such as colon cancer, esophagus cancer, and breast cancer, and displays vital roles in many critical cellular processes (PMID: 30154451, 25321476).

Publications

Species	Application	Title
human	WB	J Orthop Surg Res; Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes; Authors - Longfei Li
human	IF	Oncogene; Cancer-associated fibroblasts promote EGFR-TKI resistance via the CTHRC1/glycolysis/H3K18la positive feedback loop; Authors - Chen Zhang
mouse	WB,IHC	Bioact Mater; Immune-modulated adhesive hydrogel for enhancing osteochondral graft adhesion and cartilage repair; Authors - Jiaqi Zhou
mouse	WB	Inflammation; Calcified Cartilage Zone Remodeling Induced by IL-1β Derived from Necrotic Subchondral Bone Initiates Cartilage Degeneration in Patients with Glucocorticoids-induced Osteonecrosis of the Femoral Head; Authors - Pengbo Wang
mouse	IHC	Calcif Tissue Int; Mineral Content and Extracellular Matrix Protein Expression in Mouse Growth Plates During Epiphyseal Fusion: An Observational Study; Authors - Xinhang Yu

{{ptg:RelatedPrimaryAntibodies}}