Anticorps Polyclonal de lapin anti-Collagen Type XVI

Collagen Type XVI Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 19802-1-AP

Synonymes

COL16A1, COL16, FP1572, Collagen alpha-1(XVI) chain, Collagen alpha 1(XVI) chain

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Galerie de données de validation

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  WB analysis of human placenta using 19802-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19802-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skin using 19802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Collagen Type XVI antibody (19802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain
• Résultats positifs en IF-P: tissu cutané de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

19802-1-AP cible Collagen Type XVI dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: collagen, type XVI, alpha 1
• Masse moléculaire calculée: 158 kDa
• Poids moléculaire observé: 158 kDa
• Numéro d’acquisition GenBank: NM_001856
• Symbole du gène: COL16A1
• Identification du gène (NCBI): 1307
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COL16A1, also named as FP1572, belongs to the fibril-associated collagens with interrupted helices (FACIT) family. COL16A1 is involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology. The antibody is specific to COL16A1.

Publications

Species	Application	Title
Human	WB	Nat Commun; TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer.; Authors - Rajat Banerjee
human	WB	Aging (Albany NY); MicroRNA-152 and -181a participate in human dermal fibroblasts senescence acting on cell adhesion and remodeling of the extra-cellular matrix.; Authors - Mancini Mara M

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